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1. Bovine serum is one of the traditional Chinese medicine ingredients in cell culture medium. Adding appropriate amount of serum (10%) to the medium can first supplement the growth factors, hormones, and attachment factors required for cell growth.
2, has a detoxification effect, can relieve the toxicity of fatty acids, heavy metals and certain proteases.
3. It also stops the action of pancreatic enzymes and protects cells from mechanical damage. However, the serum components are complex, and the serum produced by different sources and different batches has a great difference in cell growth. The shelf life of the serum is only one year, and the sera of different storage periods are also different. Therefore, when the newly purchased serum is to start a major trial, the screening of bovine serum should be carried out first.
Why do you choose bovine serum for cell culture experiments?
Serum is a complex mixture of fibrin removed from plasma. Serum composition and content often vary with the sex, age, physiological conditions and nutritional conditions of the blood donor. The serum contains various plasma proteins, peptides, fats, carbohydrates, growth factors, hormones, inorganic substances, etc., which are physiologically balanced for promoting cell growth or inhibiting growth activity. The reasons why researchers choose to use bovine serum for cell experiments are as follows: