PCR ( Polymerase Chain Reaction)
Superyears General 2215 RT-qPCRadopts all-in-one with an industrial art design, combining the Analytical Instrument, Large touch screen, and Computer. General 2215 RT-qPCR is equipped with the proven thermal-cooled electric technology, which can recognize the speed rate of temperature control faster and obtain high-quality testing results within 40 minutes. To ensure the sensitivity and stability of the testing a design with traditional beam path with a susceptible CMOS image sensor is used. In addition, General 2215 supports multiple PCR analysis and High-resolution Melt Curve (HRM) analysis, compatible with the mainstream reagent kit in the market. It will be the best assistant and your preferred RT-qPCR.
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It provides a way to make more copies of a portion of DNA.
We need to have PCR ingredients that include DNA portion, Buffer solution, Primer+DNA (tap polymerase), and Nucleotides during the preparation step.
In the step of PCR sequence, there are three steps in this section.
Step 1 Denaturation
The addition of heat is needed to separate the two strands of the DNA molecule.
Step 2 Annealing
Temperature, is going to be cool in this step, should allow the primers to bind to the specific segments.
Step 3 DNA Synthesis
To make more copies of the DNA.
Those three steps above keep repeating to make enough copies of the DNA fragments. It is the principle of how PCR machine works.
Crusher - use and maintenance
1. Characteristics of the crusher
1. Wide application range, the whole machine is made of stainless steel material, which can meet the requirements of many industries. The crushing room is equipped with water-cooled jacket to meet the crushing requirements of most heat sensitive materials.
2. No pollution, low noise, separate assembly and dust collection chambers, so that the dust can be fully collected to meet the user's requirements for cleanliness; the overall balance of the maneuver is good, and the noise is greatly reduced.
3. Strong production capacity, which can meet the production requirements of most users.
2. Grinder use and maintenance
1. Before using the pulverizer, check whether the shape of the machine is damaged or not, and whether the random data is complete.
Before turning on the power to the pulverizer, please check if there are any foreign objects in the machine cavity to prevent the knife from being punched and manually turn the flywheel more than one turn. It is absolutely forbidden to move the knife by hand to ensure safety.
Make sure that the main machine of the shredder is turned correctly and the motor can be started and put into production.
Note: The main machine of the shredder should not be reversed when it is running. Otherwise, serious equipment accidents such as damage to the fixed and fixed knives and damage to the left and right labyrinth will result.
2. The pulverizer depends on the material characteristics. The normal use time has its own length. If there is wear, please use the blade. When installing the knife, the rotary knife must be fastened. After the fixed knife is only needed and fixed slightly, adjust the 4 top knives. Cut the screws to ensure that the rotating knife and the fixed knife maintain a clearance of 0.2-0.3. Then tighten the fixing knife screw and tighten the adjusting screw to complete the knife installation.
The crusher runs for about 500 hours. Please check the left and right main bearings. If necessary, clean them, add appropriate amount of grease or replace all grease.
The working load of the crusher is heavier, and it is necessary to check the tightening of the transmission triangle tape frequently to ensure smooth and reliable transmission of the movable knife.
Excerpt from "Mechanical Expert Network"